Differential Expression Profiles and Functional Prediction of tRNA-Derived Small RNAs in Rats After Traumatic Spinal Cord Injury.

摘要: Spinal cord injury (SCI) is mostly caused by trauma. As the primary mechanical unavoidable, a focus on underlying molecular mechanisms of SCI-induced secondary necessary to develop promising treatments for patients with SCI. Transfer RNA-derived small RNA (tsRNA) novel class short, non-coding RNA, possessing potential regulatory functions in various diseases. However, functional roles tsRNAs traumatic SCI have not been determined yet. We used combination sequencing, quantitative reverse transcription-polymerase chain reaction (qRT-PCR), bioinformatics, and luciferase reporter assay screen expression profiles identify after result, 297 differentially expressed were identified rats' spinal 1 day contusion. Of those, 155 significantly expressed: 91 up-regulated, whereas 64 down-regulated (fold change > 1.5; P < 0.05). Bioinformatics analyses revealed candidate (tiRNA-Gly-GCC-001, tRF-Gly-GCC-012, tRF-Gly-GCC-013, tRF-Gly-GCC-016) that might play through mitogen-activated protein kinase (MAPK) neurotrophin signaling pathways targeting brain-derived neurotrophic factor (BDNF). validated found opposite trends levels BDNF Finally, tiRNA-Gly-GCC-001 was target using assay. In summary, we an altered tsRNA pattern predicted be involved MAPK BDNF, thus regulating post-SCI pathophysiologic processes. This study provides insights future investigations explore therapeutic targets