Efficient aerobic succinate production from glucose in minimal medium with Corynebacterium glutamicum.

摘要: Corynebacterium glutamicum, an established industrial amino acid producer, has been genetically modified for efficient succinate production from the renewable carbon source glucose under fully aerobic conditions in minimal medium. The initial deletion of dehydrogenase genes (sdhCAB) led to accumulation 4.7 g l(-1) (40 mM) as well high amounts acetate (125 by-product. By deleting all known acetate-producing pathways (pta-ackA, pqo and cat) could be strongly reduced by 83% increased up 7.8 (66 mM). Whereas overexpression glyoxylate shunt (aceA aceB) or overproduction anaplerotic enzyme pyruvate carboxylase (PCx) had only minor effects on production, simultaneous PEP resulted a strain that produced 9.7 (82 with specific productivity 1.60 mmol (cdw)(-1) h(-1). This value represents highest among currently described bacterial producers. Optimization decoupling cell growth using most advanced producer (C. glutamicumΔpqoΔpta-ackAΔsdhCABΔcat/pAN6-pyc(P458S) ppc) additional increase product yield 0.45 mol mol(-1) titre 10.6 (90 succinate.