Comparative 2D-DIGE analysis of salinity responsive microsomal proteins from leaves of salt-sensitive Arabidopsis thaliana and salt-tolerant Thellungiella salsuginea

摘要: Abstract Halophytes have evolved unique molecular strategies to overcome high soil salinity but we still know very little about the main mechanisms that these plants use complete their lifecycle under stress. One useful approach further our understanding in this area is directly compare response of two closely related species which show diverse levels salt tolerance. Here present a comparative proteomic study using DIGE leaf microsomal proteins identify salt-responsive membrane associated Arabidopsis thaliana (a glycophyte) and Thellungiella salsuginea halophyte). While small number distinct protein abundance changes were observed upon stress both species, most notable differences between specifically, untreated with total 36 displaying significant changes. Gene ontology (GO) term enrichment analysis showed majority distributed into functional categories; transport (31%) carbohydrate metabolism (17%). Results several novel responsive system support theory T. shows degree salt-tolerance because are primed deal This intrinsic ability anticipate distinguishes it from glycophyte A. thaliana. Biological significance There interest tolerate as salinization becoming an increasing concern for agriculture Na+ leading reduced yields economic loss. Much knowledge on employed by combat has come work salt-sensitive plants, studies naturally occurring highly salt-resistant halophytes, direct comparisons glycophytes could help tolerance mechanisms. In study, employing differ markedly salt-tolerance, carried out quantitative 2D-DIGE contrast plant species. Our complements previous iTRAQ technology (34) highlights benefits alternative technologies approaches gain broader representation proteome article part Special Issue entitled: Proteomics, mass spectrometry peptidomics, Cancun 2013. Guest Editors: Cesar Lopez-Camarillo, Victoria Pando-Robles Bronwyn Jane Barkla.