Anti-HIV-1 activity of recombinant and hybrid species of interferon-α
作者： STEVEN J. SPERBER , DAVID J. GOCKE , CECILIA HABERZETTL , REBECCA KUK , BARBARA SCHWARTZ
关键词: Virology 、 Biological activity 、 Virus 、 Interferon alfa 、 Alpha interferon 、 Vesicular stomatitis virus 、 In vitro 、 Viability assay 、 Biology 、 Recombinant DNA
摘要: ABSTRACT To identify candidate interferons (IFNs) for the treatment of human immunodeficiency virus type 1 (HIV-1) infection and to investigate sequence–function relationships, antiviral activities nine species recombinant IFN-α [IFN-αA, IFN-αB, IFN-αC, IFN-αD, IFN-αJ, [Ser116]IFN-αJ1, IFN-αK, IFN-αJ/C(Fnu4HI), IFN-αA/D(BglII)] were evaluated against HIV-1. MT-2 cells exposed various concentrations each IFN then infected with HIV. Protective effect was determined by cell viability using a tetrazolium dye assay. Activity vesicular stomatitis (VSV) assessed on MDBK WISH cells. The 50% inhibitory concentration HIV 37 ± 14 pg/ml IFN-αA, ranged from 15 3 IFN-αJ/C(Fnu4HI) >90,000 IFN-αD. In general, relative activity similar VSV IFN-αD notable its decreased observations suggest that it may be possible produce IFNs...
liebertonline.com参考文章(24)
Sidney Pestka, [1] Interferon from 1981 to 1986 Methods in Enzymology. ,vol. 119, pp. 3- 14 ,(1986) , 10.1016/0076-6879(86)19003-3
Jerome A. Langer, Sidney Pestka, Procedures for studying binding of interferon to human cells in suspension cultures. Methods in Enzymology. ,vol. 119, pp. 305- 311 ,(1986) , 10.1016/0076-6879(86)19046-X
E Rehberg, B Kelder, E G Hoal, S Pestka, Specific molecular activities of recombinant and hybrid leukocyte interferons. Journal of Biological Chemistry. ,vol. 257, pp. 11497- 11502 ,(1982) , 10.1016/S0021-9258(18)33788-8
J R Ortaldo, A Mason, E Rehberg, J Moschera, B Kelder, S Pestka, R B Herberman, Effects of recombinant and hybrid recombinant human leukocyte interferons on cytotoxic activity of natural killer cells. Journal of Biological Chemistry. ,vol. 258, pp. 15011- 15015 ,(1983) , 10.1016/S0021-9258(17)43764-1
S Rubinstein, P C Familletti, S Pestka, Convenient assay for interferons. Journal of Virology. ,vol. 37, pp. 755- 758 ,(1981) , 10.1128/JVI.37.2.755-758.1981
G C Sen, R Herz, V Davatelis, S Pestka, Antiviral and protein-inducing activities of recombinant human leukocyte interferons and their hybrids. Journal of Virology. ,vol. 50, pp. 445- 450 ,(1984) , 10.1128/JVI.50.2.445-450.1984
D S Hobbs, S Pestka, Purification and characterization of interferons from a continuous myeloblastic cell line. Journal of Biological Chemistry. ,vol. 257, pp. 4071- 4076 ,(1982) , 10.1016/S0021-9258(18)34687-8
Philip C. Familletti, Sara Rubinstein, Sidney Pestka, A convenient and rapid cytopathic effect inhibition assay for interferon. Methods in Enzymology. ,vol. 78, pp. 387- 394 ,(1981) , 10.1016/0076-6879(81)78146-1
B. L. Li, J. A. Langer, B. Schwartz, S. Pestka, Creation of phosphorylation sites in proteins: construction of a phosphorylatable human interferon alpha Proceedings of the National Academy of Sciences of the United States of America. ,vol. 86, pp. 558- 562 ,(1989) , 10.1073/PNAS.86.2.558
Ronald Rooke, Michel Tremblay, Hugo Soudeyns, Lucie DeStephano, Xiao-Jian Yao, Mary Fanning, Julio S.G. Montaner, Michael OʼShaughnessy, Karen Gelmon, Chris Tsoukas, John Gill, John Ruedy, Mark A. Wainberg, Isolation of drug-resistant variants of HIV-1 from patients on long-term zidovudine therapy. Canadian Zidovudine Multi-Centre Study Group. AIDS. ,vol. 3, pp. 411- 416 ,(1989) , 10.1097/00002030-198907000-00001