Production of α-ketoisocaproate via free-whole-cell biotransformation by Rhodococcus opacus DSM 43250 with l-leucine as the substrate
作者： Yuhong Zhu , Jianghua Li , Long Liu , Guocheng Du , Jian Chen
DOI: 10.1016/J.ENZMICTEC.2011.06.008
关键词: Chemistry 、 Response surface methodology 、 Substrate (chemistry) 、 Biotransformation 、 Chromatography 、 Substrate concentration 、 Cell concentration 、 Rhodococcus opacus 、 Whole cell 、 Leucine 、 Biochemistry
摘要: Abstract This work aims to produce α-ketoisocaproate (KIC) from l -leucine via the free-whole-cell biotransformation of Rhodococcus opacus DSM 43250. The effects temperature, pH, substrate concentration, cell and rotating speed on KIC production were examined. Furthermore, conditions optimized with response surface methodology (RSM). optimal as follows: temperature 43.7 °C, pH 8.4, concentration 5.1 g/L, 30.4 g/L, 170 rpm. maximal predicted by RSM model reached 1275 mg/L at conditions, in validated experiments, 1264 mg/L, which was very close production. current provides an alternative for results obtained here are useful biotransformatic a large scale.
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sci-hub.se 参考文章(19)
M. E. Tischler, A. L. Goldberg, Amino acid degradation and effect of leucine on pyruvate oxidation in rat atrial muscle American Journal of Physiology-endocrinology and Metabolism. ,vol. 238, ,(1980) , 10.1152/AJPENDO.1980.238.5.E480
B. Chua, D.L. Siehl, H.E. Morgan, Effect of leucine and metabolites of branched chain amino acids on protein turnover in heart. Journal of Biological Chemistry. ,vol. 254, pp. 8358- 8362 ,(1979) , 10.1016/S0021-9258(19)86898-9
Kanji Hori, Keisuke Miyazawa, Keiji Ito, Purification and some properties of L-amino acid oxidase from Amphiroa crassissima Yendo Hydrobiologia. ,vol. 151, pp. 563- 569 ,(1987) , 10.1007/978-94-009-4057-4_83
Ralph E. Kirsch, Lesley O'C. Frith, Stuart J. Saunders, Stimulation of albumin synthesis by keto analogues of amino acids Biochimica et Biophysica Acta. ,vol. 442, pp. 437- 441 ,(1976) , 10.1016/0005-2787(76)90317-8
Iris Hilker, Chris Baldwin, Véronique Alphand, Roland Furstoss, John Woodley, Roland Wohlgemuth, On the influence of oxygen and cell concentration in an SFPR whole-cell biocatalytic Baeyer-Villiger oxidation process Biotechnology and Bioengineering. ,vol. 93, pp. 1138- 1144 ,(2006) , 10.1002/BIT.20829
Die Hu, Zhenyu Shi, Qiong Wu, Guo-Qiang Chen, None, Microbial production of acetoacetate by recombinant Escherichia coli Bioresource Technology. ,vol. 101, pp. 8477- 8480 ,(2010) , 10.1016/J.BIORTECH.2010.06.021
V. Leclercq-Meyer, J. Marchand, R. Leclercq, W. J. Malaisse, Interactions of α-ketoisocaproate, glucose and arginine in the secretion of glucagon and insulin from the perfused rat pancreas Diabetologia. ,vol. 17, pp. 121- 126 ,(1979) , 10.1007/BF01222213
Prabhjot Kaur Gill, Rajesh Kumari Manhas, Prabhjeet Singh, Hydrolysis of inulin by immobilized thermostable extracellular exoinulinase from Aspergillus fumigatus Journal of Food Engineering. ,vol. 76, pp. 369- 375 ,(2006) , 10.1016/J.JFOODENG.2005.05.052
G. M. Brearley, C. P. Price, T. Atkinson, P. M. Hammond, Purification and partial characterisation of a broad-range L-amino acid oxidase from Bacillus carotarum 2Pfa isolated from soil Applied Microbiology and Biotechnology. ,vol. 41, pp. 670- 676 ,(1994) , 10.1007/BF00167283
Jonathan S. Nimitz, Harry S. Mosher, A new synthesis of .alpha.-keto esters and acids Journal of Organic Chemistry. ,vol. 46, pp. 211- 213 ,(1981) , 10.1021/JO00314A057