Progress in Monolithic Column-based Separation and Enrichment of Glycoproteins
作者： Xue WEN , Hai-Jiao ZHENG , Yang ZHANG , Qiong JIA
DOI: 10.1016/S1872-2040(19)61207-7
关键词: Protein glycosylation 、 Monolithic HPLC column 、 Drug treatment 、 Pretreatment method 、 Glycoprotein 、 Chemistry 、 High complexity 、 Nanotechnology
摘要: Abstract As a post-translational modification, protein glycosylation not only reflects on body health, but also serves as the target of drug treatment. Therefore, it is great significance to study for diagnosis and treatment diseases. However, many glycoproteins biological importance exhibit relatively low abundance, high complexity, wide dynamic range in samples. It indispensable explore highly efficient sample pretreatment methods. In recent years, monolithic columns have attracted extensive attention field separation enrichment due their rapid separation, efficiency, good compatibility. this paper, progress surveyed, future development prospected.
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