Rv0216, a conserved hypothetical protein fromMycobacterium tuberculosisthat is essential for bacterial survival during infection, has a double hotdog fold
作者： Alina Castell , Patrik Johansson , Torsten Unge , T. Alwyn Jones , Kristina Bäckbro
DOI: 10.1110/PS.051442305
关键词: Biochemistry 、 Gene product 、 Gene 、 Peptide sequence 、 Mycobacterium tuberculosis 、 Protein structure 、 Hypothetical protein 、 Conserved sequence 、 Genetics 、 Biology 、 Genome 、 Molecular biology
摘要: The Mycobacterium tuberculosis genome contains about 4000 genes, of which approximately a third code for proteins unknown function or are classified as conserved hypothetical proteins. We have determined the three-dimensional structure one these, rv0216 gene product, has been shown to be essential M. growth in vivo. exhibits greatest similarity bacterial and eukaryotic hydratases that catalyse R-specific hydration 2-enoyl coenzyme A. However, only part catalytic machinery is Rv0216 it showed no activity substrate crotonyl-CoA. allows us assign new functional annotations family seven other proteins, number if survival during infection growth.
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