The 4-Hydroxylation of Cinnamic Acid by Sorghum Microsomes and the Requirement for Cytochrome P-450
作者： J. Rowell M. Potts , Richard Weklych , Eric E. Conn
DOI: 10.1016/S0021-9258(19)42322-3
关键词: Cinnamic acid 、 Catalysis 、 Hydroxylation 、 Biochemistry 、 Microsome 、 Monooxygenase 、 Cytochrome 、 Chemistry 、 Action spectrum 、 Carbon monoxide
摘要: Cinnamic acid 4-hydroxylase in etiolated sorghum seedlings was concentrated the microsomal or light membrane fraction upon extraction. The three substrates required catalysis, cinnamic acid, TPNH, and O2 have Km values micromolar range. same contained cytochromes P-450 b5 at 0.10 0.55 nmoles · mg of protein-1, respectively. Preparation a photodissociation action spectrum on carbon monoxide inhibition hydroxylase indicated requirement for cytochrome catalysis. When challenged with range potential inhibitors, behaved manner that is fairly typical more extensively studied monooxygenases nonplant tissues.
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