Quantitative PCR shows propagation of Plasmodiophora brassicae in Swedish long term field trials
作者： Jonsson Anders , Marzec-Schmidt Katarzyna , Börjesson Gunnar , Wallenhammar Ann-Charlotte
DOI: 10.1007/S10658-016-0906-X
关键词: Crop 、 Phosphorus 、 Biology 、 Crop rotation 、 Potassium 、 Clubroot 、 Agronomy 、 Soil test 、 Brassica 、 Soil horizon
摘要: Clubroot (Plasmodiophora brassicae) is a serious soil-borne disease in brassica crops world-wide. We report on time series of soil samples from Swedish long-term fertility trials started 1957, 1963 and 1966, which were analyzed for the amount ofP. brassicaeDNA. The crop rotations included every 4 or 6years. All experimental sites with 4-year rotation oilseed rape, except one calcium carbonate profile, showed high (>1000fg DNA g−1soil) levels brassicaeDNA after 9, 11 12 rotations. In contrast, detectable (>5fg brassicaewere found only at five 6-year spring rape. years brassicaeDNA, low yield was reported subsequent decline inP. observed. Different NPK (nitrogen/phosphorus/potassium) fertiliser regimes resulted similarP. levels. robustness reliability method applied verified by analyses individual plots compared mixture repeated selected samples, thatP. remained stable during dry storage.
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