MiR-483-3p inhibition ameliorates myocardial ischemia/reperfusion injury by targeting the MDM4/p53 pathway
作者： Haishan Zhang , Jia Wang , Aolin Du , Yang Li
DOI: 10.1016/J.MOLIMM.2020.06.014
关键词: Viability assay 、 Apoptosis 、 Lactate dehydrogenase 、 Myocardial ischemia 、 Chemistry 、 Pharmacology 、 Downregulation and upregulation 、 Pathogenesis 、 Reperfusion injury 、 Transfection
摘要: MiR-483-3p is involved in the pathogenesis of acute myocardial infarctions, but its association with ischemia reperfusion (IR) remains mostly unknown. In this study, an vitro model IR injury was established by putting H9c2 cells into hypoxia reoxygenation (HR) treatment to explore effects and possible mechanisms miR-483-3p injury. HR exposure resulted increased levels cells. overexpressed or downregulated transfection mimic inhibitor. HR-treated cells, mimics inhibited cell viability, promoted lactate dehydrogenase release, apoptosis, inhibitors caused opposite effects. MDM4 verified be target mRNA negatively modulated miR-483-3p. inhibitor upregulated Bcl-2, p53 Bax whereas overexpression produced effects.. Moreover, siRNA partially reversed role inhibition pathway inactivation summary, targeting MDM4/p53 pathway, may alleviate a potential therapeutic
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