A U1 snRNA:pre-mRNA base pairing interaction is required early in yeast spliceosome assembly but does not uniquely define the 5' cleavage site.

摘要: Abstract We analyzed the effects of suppressor mutations in U1 snRNA (SNR19) gene from Saccharomyces cerevisiae on splicing mutant pre-mRNA substrates. The results indicate that pairing between and highly conserved position 5 (GTATGT) intron occurs early spliceosome assembly vitro. This is important for efficient both vitro vivo. However, at does not appear to influence 5' splice site selection vivo, indicating previously described snRNA:5' junction base interaction sufficient define cleavage site.