Platelet membrane glycoprotein IIIa contains target antigens that bind anti-platelet antibodies in immune thrombocytopenias.

摘要: Abstract The precise pathogenic mechanism of platelet destruction in immune thrombocytopenias is not known, although many investigators have found that platelet-associated IgG increased these diseases. We report here the differentiation between specific binding anti-platelet antibody, associated with destruction, and ubiquitous presence nonspecific, IgG. Using an electrophoretic separation antibody overlay technique, we identified a membrane protein bears target antigens thrombocytopenias. When posttransfusion purpura serum was studied, to PlA1 antigen on glycoprotein IIIa readily distinguished from nonspecific immunoglobulin 200,000 mol wt. After reduction disulfide bonds, antigenicity observed, bound nonspecifically band apparent molecular weight 45,000. also antibodies patients idiopathic thrombocytopenic determined their antigenic specificity. Antibodies which bind 100,000-mol wt were nine thirteen chronic disease. The three cases studied detail by using both reduced nonreduced control Glanzmann's thrombasthenic platelets. Target localized IIIa, but are different PlA1. system clearly Sera eight children acute studied. In all cases, 200,000-mol observed. However, unable demonstrate suggested childhood form this disease may than autoimmune cases.