Inheritance of GFP-Bt transgenes from Brassica napus in backcrosses with three wild B. rapa accessions.

摘要: Transgenes from transgenic oilseed rape, Brassica napus (AACC genome), can introgress into populations of wild B. rapa (AA but little is known about the long-term persistence transgenes different transformation events. For example, that are located on crop’s C chromosomes may be lost during process introgression. We investigated genetic behavior in backcross generations after nine GFP (green fluorescent protein)-Bt ( Bacillus thuringiensis ) B. napus lines, named GT were hybridized with three accessions, respectively. Each generation involved crosses between hemizygous plants and non-GT pollen recipients. In some cases, sample sizes too small to allow detection major deviations Mendelian segregation ratios, GT:non-GT was consistent an expected ratio 1:1 all BC 1 generation. Starting 2 generation, significantly observed among lines. showed a , 3 4 generations. However, other lines deviated generations, which had fewer progeny than expected, not Most importantly, two did fit or due deficiency progeny. these strong reduction transgene introgression accessions. These findings imply genomic location affect hybridization has occurred.