Facile synthesis of human insulin by modification of porcine insulin

摘要: A method of converting natural porcine insulin to human by reversibly blocking the six carboxyl groups in insulin, digesting blocked with trypsin remove terminal octapeptide from B chain and generating a free group arginine residue position 22 chain, then all amino preferably BOC azide coupling at synthetic which corresponds sequence end finally removing protecting purifying recovering insulin. This is also susceptible preparing radioactively labelled utilizing radioactive acids synthesis grafted or placed on residue. Further present permits facile analogs, since any selected acid may be attached 22. The above method, summary, means chemical enzymatic treatment, makes possible removal quantitatively selectively chain. fragment replaced an analogous give protein identical satisfying number criteria.