Differential actions of isoflurane and ketamine-based anaesthetics on cochlear function in the mouse.

摘要: Isoflurane is a volatile inhaled anaesthetic widely used in animal research, with particular utility for hearing research. has been shown to blunt sensitivity compared the awake state, but little known about how isoflurane compares other anaesthetics regard hair cell transduction and auditory neurotransmission. The current study was undertaken C57Bl/6J C129/SvEv strains of mice determine whether anaesthesia affects function relative ketamine-based anaesthesia. Cochlear central transmission were assessed using brainstem response (ABR) distortion product otoacoustic emission (DPOAE), comparing thresholds input/output functions over time, vs. ketamine/xylazine/acepromazine ABR at most sensitive region (16 kHz) initially higher under This reduced worsened 1 h period, also became evident broadband click stimulus. Ketamine provided stable thresholds. Although growth unchanged time both anaesthetics, slopes significantly less. Cubic (2f1–f2) DPOAE similar anaesthetics. After 60 min, increased groups, this effect greater ketamine anaesthesia. The isoflurane-mediated increase attributable action on cochlear nerve activation, as right-shift P1-N1 K/X/A. produced gain despite outer – mediated function.