作者: Maarit Bärlund , Outi Monni , Anne Kallioniemi , Päivikki Kauraniemi
DOI:
关键词: Biology 、 Comparative genomic hybridization 、 Genetics 、 Gene duplication 、 Chromosomal region 、 Molecular biology 、 Complementary DNA 、 Amplicon 、 Expressed sequence tag 、 DNA microarray 、 Copy-number variation
摘要: Amplification of the ERBB2 oncogene at 17q12 has been well documented in breast cancer and has been shown to contribute to a poor clinical outcome. However, systematic surveys of copy number and expression levels of all genes within the 17q12 region have not been performed. Here, we used cDNA and comparative genomic hybridization microarray technologies to undertake a broad survey of genes involved in the 17q12 amplification in breast cancer. A chromosomal region-specific cDNA microarray containing 217 expressed …