Salt stress affects glutamine synthetase activity and mRNA accumulation on potato plants in an organ-dependent manner

摘要: Ammonium assimilation into glutamine and glutamate is vital for plant growth as these are precursors almost all nitrogenous compounds. can be assimilated onto organic compounds by the concerted action of two enzymes that compose synthetase (GS, EC 6.3.1.2) - synthase (Fd-GOGAT, 1.4.7.1; NADH-GOGAT, 1.4.1.14) cycle. may also directly incorporated dehydrogenase (GDH, 1.4.1.2) aminating reaction. However, GDH reversibly deaminates glutamate, its physiological role in vivo remains controversial. Potato has been classified moderately tolerant to salinity. GS encoded a small multigene family which differentially regulated an organ age-dependent way. In this study, effect increasing concentrations salinity soil activity gene-specific mRNA accumulation levels were studied on potato leaves roots, well biochemical parameters protein, chlorophyll, lipid peroxidation proline levels, order evaluate severity imposed stress. The data obtained suggests when plants subjected salt stress, increased ammonium occurs due accumulation, along with decreased leaves. Regarding organ-dependent response was observed contributes detected alteration assimilatory metabolism. This key feature future genetic manipulations increase crop productivity salty soils. possible contribution ammonia investigated.