Characterization of Two Novel Mutants of DNA Polymerase Delta in Drosophila melanogaster

摘要: DNA is the genetic material for all living organisms which constantly being unpackaged replicated and repackaged. The replication of this involves numerous different proteins; however polymerase delta (pol [delta]) carries much load by replicating a major portion genome in both leading lagging strand synthesis. Using model organism Drosophila melanogaster we investigate two novel mutations evolutionary conserved regions. One region corresponds with polymerase's ability to polymerize new nucleotides onto an existing DNA. other proofread 3' 5' direction. These mutants homozygous lethal recessive show interesting phenotypes delay S-phase chromosome aberrations defects endoreplication possible protection form damage. these domains can be further characterized. By understanding how pol [delta] functions vivo setting apply knowledge mechanics cancer biology humans another multicelluar inform therapies treat it.