Assessment of somaclonal variation and stability in In vitro regenerated grasspea plants using SDS-PAGE

摘要: Tissue culture may be one of the possible sources variation for crop improvement. To assess and stability in regenerated plants, shoots were from callus derived axillary explants 11 grasspea genotypes, their shoot protein profiles compared with those seed cultured plants. The highest response induction (87%) was observed when 2.0 mg/l2,4-dichlorophenoxyacetic acid(2,4-D) 0.25 mg/l BAP supplemented Murashige Skoog’s (MS) medium. a-naphthaleneacetic acid(NAA) 6-benzylaminopurine(BAP) showed variable redifferentiation along formation. MS medium 0.5 better multiplication elongation shoots.Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) analysis a unique band 43 kDa both tissue plants Pusa 24. Polypeptide banding pattern genotypes did not deviate parental protein. Similarity coefficient values ranged 0.37 to 0.85 mean 0.43 among 55 genotypic combinations. Comparison bands between calli raised seeds revealed absence somaclonal suggesting that regeneration protocol used present study can genomics enabled improvement without risk additional or instability.