Nanoprobes for Live-Cell Gene Detection
作者： Gang Bao , Won Jong Rhee , Andrew Tsourkas
DOI: 10.1007/978-1-4419-0040-1_14
关键词: Gene expression 、 Molecular beacon 、 Oligomer restriction 、 Oligonucleotide 、 RNA 、 DNA microarray 、 Gene 、 Biology 、 Fluorescence in situ hybridization 、 Computational biology
摘要: The ability to visualize in real time the expression dynamics and localization of specific RNAs vivo offers tremendous opportunities for biological disease studies including cancer detection. However, quantitative methods such as real-time polymerase chain reaction (PCR) DNA microarrays rely on use cell lysates thus not able obtain important spatial temporal information. Fluorescence proteins other reporter systems cannot image endogenous RNA living cells. situ hybridization (FISH) assays require washing achieve specificity, therefore can only be used with fixed Here we review recent development nanostructured probes detection discuss engineering issues challenges quantifying gene vivo. In particular, describe that oligonucleotide probes, combined novel delivery strategies, relative level, localization, live Examples detecting messenger RNAs, well imaging their subcellular are given illustrate applications, probe design, delivery, target accessibility discussed. promise open new exciting sensitive a wide range medical applications.
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