A monoclonal antibody that appears to recognize the receptor for human T-cell growth factor; partial characterization of the receptor

摘要: T-cell growth factor (TCGF or interleukin-2) is an inducible glycoprotein hormone of molecular weight 15,000 (ref. 1) synthesized and secreted by T lymphocytes following activation with antigen mitogen2,3. TCGF required for proliferation expansion cells encounter4,5 to maintain them in long-term culture vitro6–9. Full expression the human immune response requires both induction synthesis formation specific membrane receptors10,11. Monoclonal antibodies binding have been prepared12,13. In contrast, receptor not identified, nor has characterized. We prepared a monoclonal antibody, termed anti-Tac14,15, which appears bind TCGF. support this, we now demonstrate that anti-Tac suppresses induced blocks radiolabelled from cloned continuous line. Also partially purified characterized putative receptor. This (Mr) 47,000–53,000.