Mass spectroscopy analysis of mutant polypeptides in biological samples
作者： Roberto Polakiewicz , Albrecht Moritz , Edward Rush Ii John
DOI:
关键词: Hydrolysis 、 Mass spectrometry 、 Molecule 、 Mutant 、 Chromatography 、 Chemistry 、 Elution 、 Peptide 、 Substrate (chemistry)
摘要: The invention relates to a method for determining the presence of at least one distinct polypeptide in biological sample comprising contacting with hydrolyzing agent, wherein agent is capable sequence-specific manner such that peptide having predetermined measured accurate mass would result if were present sample, obtain hydrolyzed sample; bringing contact substrate immobilized binding partner, partner specifically peptide; removing from remain bound partner; an elution solution, dissociate into solution; subjecting portion solution liquid chromatography segregate plurality molecules sorted molecules; molecule and when substantially equal mass.
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