The dps promoter is activated by OxyR during growth and by IHF and σs in stationary phase
作者： Shoshy Altuvia , Marta Almiron , Gjalt Huisman , Roberto Kolter , Gisela Storz
DOI: 10.1111/J.1365-2958.1994.TB00421.X
关键词: Cell biology 、 Enterobacteriaceae 、 Transcription (biology) 、 Escherichia coli 、 Molecular biology 、 Hydrogen peroxide 、 Escherichia 、 Biology 、 Activator (genetics) 、 Gene expression 、 Messenger RNA
摘要: Summary Dps is a non-specific DNA-binding protein abundant In starved Escherichia eoii ceiis and important for the defence against hydrogen peroxide. We found that dps mRNA leveis are controiied by rpoS-encoded a^, transcriptionai activator OxyR histoneiike iHF protein, in exponentiaily growing ceiis, induced treatment with peroxide an OxyR-dependen t manner. This OxyR-dependent induction occurs oniy during iog phase, aithough present stationary phase ceits, expressed a^- iHF-dependent The purified proteins aiso shown to bind upstream of promoter. Our results suggest promoter recognized both o^°-hoioenzyme
sci-hub.se 参考文章(26)
Michael F. Christman, Robin W. Morgan, Fredric S. Jacobson, Bruce N. Ames, Positive Control of a Regulon for Defenses against Oxidative Stress and Some Heat-Shock Proteins in Salmonella typhimurium Cell. ,vol. 41, pp. 753- 762 ,(1985) , 10.1016/S0092-8674(85)80056-8
Bruce Demple, Regulation of bacterial oxidative stress genes. Annual Review of Genetics. ,vol. 25, pp. 315- 337 ,(1991) , 10.1146/ANNUREV.GE.25.120191.001531
Gisela Storz, Shoshy Altuvia, [17] OxyR regulon Methods in Enzymology. ,vol. 234, pp. 217- 223 ,(1994) , 10.1016/0076-6879(94)34088-9
R Lange, M Barth, R Hengge-Aronis, Complex transcriptional control of the sigma s-dependent stationary-phase-induced and osmotically regulated osmY (csi-5) gene suggests novel roles for Lrp, cyclic AMP (cAMP) receptor protein-cAMP complex, and integration host factor in the stationary-phase response of Escherichia coli. Journal of Bacteriology. ,vol. 175, pp. 7910- 7917 ,(1993) , 10.1128/JB.175.24.7910-7917.1993
S Mukhopadhyay, H E Schellhorn, Induction of Escherichia coli hydroperoxidase I by acetate and other weak acids. Journal of Bacteriology. ,vol. 176, pp. 2300- 2307 ,(1994) , 10.1128/JB.176.8.2300-2307.1994
D A Siegele, R Kolter, Life after log. Journal of Bacteriology. ,vol. 174, pp. 345- 348 ,(1992) , 10.1128/JB.174.2.345-348.1992
J A Imlay, S Linn, Bimodal pattern of killing of DNA-repair-defective or anoxically grown Escherichia coli by hydrogen peroxide. Journal of Bacteriology. ,vol. 166, pp. 519- 527 ,(1986) , 10.1128/JB.166.2.519-527.1986
Purification and characterization of the Escherichia coli OxyR protein, the positive regulator for a hydrogen peroxide-inducible regulon. Journal of Biochemistry. ,vol. 109, pp. 262- 266 ,(1991) , 10.1093/OXFORDJOURNALS.JBCHEM.A123372
S B Farr, T Kogoma, Oxidative stress responses in Escherichia coli and Salmonella typhimurium. Microbiological Research. ,vol. 55, pp. 561- 585 ,(1991) , 10.1128/MR.55.4.561-585.1991
D E Bohannon, N Connell, J Keener, A Tormo, M Espinosa-Urgel, M M Zambrano, R Kolter, Stationary-phase-inducible "gearbox" promoters: differential effects of katF mutations and role of sigma 70. Journal of Bacteriology. ,vol. 173, pp. 4482- 4492 ,(1991) , 10.1128/JB.173.14.4482-4492.1991