Hydrogen peroxide resistance in Strigomonas culicis: Effects on mitochondrial functionality and Aedes aegypti interaction.

摘要: Reactive oxygen species (ROS) are toxic molecules involved in several biological processes such as cellular signaling, proliferation, differentiation and cell death. Adaptations to oxidative environments crucial for the success of colonization insects by protozoa. Strigomonas culicis is a monoxenic trypanosomatid found midgut mosquitoes presenting life cycle restricted epimastigote form. Among S. peculiarities, there an endosymbiotic bacterium cytoplasm, which completes essential biosynthetic routes host may represent intermediary evolutive step organelle origin, thus constituting interesting model researches. In this work, we induced ROS resistance wild type epimastigotes incubation with increasing concentrations hydrogen peroxide (H2O2), compared energetic metabolisms among type, type-H2O2 resistant aposymbiotic strains. Resistant protozoa were less sensitive challenge more dependent on phosphorylation, was demonstrated higher consumption mitochondrial membrane potential, increased activity complexes II-III IV, complex II gene expression ATP production. Furthermore, strain produced reduced levels showed lower lipid peroxidation, well increase antioxidant enzymes thiol-dependent peroxidase activity. On other hand, impaired function, H2O2 production deficient response. The induction also led remarkable Aedes aegypti binding vitro vivo, indicating that both pro-oxidant environment mosquito gut stress susceptibility regulate population invertebrates.