Heme is crucial for medium-dependent metronidazole resistance in clinical isolates of C. difficile
作者： Ilse M. Boekhoud , Igor Sidorov , Sam Nooij , Céline Harmanus , Ingrid M.J.G. Bos-Sanders
DOI: 10.1101/2020.11.18.388959
关键词: Minimum inhibitory concentration 、 Biology 、 Plasmid 、 Microbiology 、 Lineage (genetic) 、 Heme 、 Gene 、 Metronidazole resistance 、 Single-nucleotide polymorphism 、 Metronidazole
摘要: Abstract Until recently, metronidazole was the first-line treatment for Clostridioides difficile infection and it is still commonly used. Though resistance has been reported due to plasmid pCD-METRO, this does not explain all cases. Here, we investigate in a collection of clinical isolates C. difficile. We find that nearly demonstrate heme-dependent increase minimal inhibitory concentration metronidazole, which some cases leads being qualified as resistant (MIC > 2 mg/L). Moreover, whole genome sequence analysis reveals single nucleotide polymorphism heme responsive gene hsmA, defines lineage PCR ribotype 010 / multilocus type 15 also includes pCD-METRO containing strains. Together our data crucial medium-dependent
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