Molecular Determinants of Enterovirus 71 Viral Entry CLEFT AROUND GLN-172 ON VP1 PROTEIN INTERACTS WITH VARIABLE REGION ON SCAVENGE RECEPTOR B 2
作者： Naiqing Xu , Yinyan Sun , Xing Wu , Xin Yao , Qunyin Mao
关键词: Viral protein 、 Capsid 、 Viral entry 、 Scavenger receptor 、 Virology 、 Receptor 、 Biology 、 Virus 、 Enterovirus 71 、 SCARB2
摘要: Enterovirus 71 (EV71) is one of the major pathogens that cause hand, foot, and mouth disease outbreaks in young children Asia-Pacific region recent years. Human scavenger receptor class B 2 (SCARB2) main cellular for EV71 on target cells. The requirements EV71-SCARB2 interaction have not been fully characterized, it has determined whether SCARB2 serves as an uncoating EV71. Here we compared efficiency from different species including human, horseshoe bat, mouse, hamster demonstrated residues between 144 151 are critical binding to viral capsid protein VP1 seven human could convert murine SCARB2, otherwise inefficient receptor, efficient infection. We also identified binds via a canyon around residue Gln-172. Soluble virions 160 S 135 particles, indicating virus. significantly increased acidic environment (pH 5.6). These studies elucidated determinants enterovirus infection revealed possible antiviral interventions.
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