The Potency of an Anti-MERS Coronavirus Subunit Vaccine Depends on a Unique Combinatorial Adjuvant Formulation.

摘要: Vaccination is one of the most successful strategies to prevent human infectious diseases. Combinatorial adjuvants have gained increasing interest as they can stimulate multiple immune pathways and enhance vaccine efficacy subunit vaccines. We investigated adjuvanticity Aluminum (alum) in combination with rASP-1, a protein adjuvant, using Middle East respiratory syndrome coronavirus MERS-CoV receptor-binding-domain (RBD) antigen. A highly enhanced anti-MERS-CoV neutralizing antibody response was induced when mice were immunized rASP-1 alum-adjuvanted RBD two separate injection sites compared + alum formulated into single inoculum. The antibodies produced also significantly inhibited binding its cell-associated receptor. Moreover, immunization co-administered resulted an frequency TfH GC B cells within draining lymph nodes, both which positively associated titers related protective immunity. Our findings not only indicate that this unique combinatorial adjuvanted regimen improved immunogenicity RBD, but point importance utilizing for induction synergistic responses.