Utilization of exogenous purine compounds in Bacillus cereus. Translocation of the ribose moiety of inosine.

摘要: Abstract Intact cells of Bacillus cereus catalyze the breakdown exogenous AMP to hypoxanthine and ribose 1-phosphate through successive action 5'-nucleotidase, adenosine deaminase, inosine phosphorylase. Inosine hydrolase was not detectable, even in crude extracts. phosphorylase causes a "translocation" moiety (as 1-phosphate) inside cell, while remains external. Even though equilibrium phosphorolytic reaction favors nucleoside synthesis, well as AMP) is almost quantitatively transformed into external hypoxanthine, since readily metabolized cell. Most likely, translocated enters sugar phosphate shunt, via its prior conversion 5-phosphate, thus supplying energy required for subsequent uptake B. cereus.