cis-Cytokinins that predominate in Pisum sativum during early embryogenesis will accelerate embryo growth in vitro

作者: Pauline E. Quesnelle , R.J. Neil Emery

DOI: 10.1139/B06-149

关键词: SativumEmbryogenesisBiologyRibosideBioassayBiochemistryCell divisionBotanyEmbryoCytokininPisum

摘要: Correlative data from monocots suggest that cytokinin (CK) regulates seed development. The involvement of CKs in growth was investigated using pea, a eudicot with an unknown CK profile, as model system. profiles were measured by liquid chromatography – tandem mass spectrometry against major stages embryogenesis, which documented histologically. Like other grain legumes, levels developing pea seeds fluctuated through development and had mainly nucleotide riboside forms. Among the 11 detected, cis-isomers (cis-[9R]Z (zeatin riboside), cis-[9RMP]Z 5′ monophosphate)), along their isopentenyl precursors, forms during whereas corresponding trans-isomers appeared minor constituents. Highest concentrations occurred at heart-shape stage, when there are high rates cell division sugar metabolism. To assess significance observed bioassay developed w...

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