Secretion of an articular cartilage proteoglycan-degrading enzyme activity by murine T lymphocytes in vitro.

摘要: Destruction of articular cartilage is the hallmark inflammatory arthritides. Enzymes elaborated by mononuclear cells infiltrating synovium mediate, in part, degradation extracellular matrix. Since are dominant cell type found chronic synovitis, we investigated whether interaction immune with antigen initiated synthesis and secretion a proteoglycan-degrading enzyme activity. Proteoglycan-degrading activity was monitored capacity murine spleen conditioned medium to release [3H]serine/35SO4 incorporated into rabbit proteoglycan monomer fraction (A1D1), relative change specific viscosity bovine nasal monomer. The results demonstrated that both virgin spontaneously generated cellular activation proliferation induced keyhole limpet hemocyanin or mitogen phytohemagglutinin not required. Kinetic studies stable over 72 h. Cell separation showed T lymphocytes, thymoma line, macrophages separately produced has been partially characterized appears belong class neutral pH metal-dependent proteinases. These observations, first demonstrate lymphocytes secrete an capable degrading proteoglycan, raise possibility this contributes matrix destruction vivo. Moreover, these data support conclusion production independent antigen-specific stimulus.