Deletion analysis of GAL4 defines two transcriptional activating segments
作者： Jun Ma , Mark Ptashne
DOI: 10.1016/0092-8674(87)90081-X
关键词: Gene expression 、 Cell biology 、 Biology 、 Yeast 、 Deletion mutant 、 Investigation methods 、 Transcription (biology) 、 Molecular biology 、 Regulation of gene expression 、 Transcriptional Activator
摘要: We describe the activities of a wide array deletion mutants GAL4, yeast transcriptional activator. identify two short regions each which activates transcription when fused to DNA-binding region molecule. Very large portions GAL4 are not required for gene activation.
sci-hub.se 参考文章(38)
J L Bennetzen, B D Hall, The primary structure of the Saccharomyces cerevisiae gene for alcohol dehydrogenase. Journal of Biological Chemistry. ,vol. 257, pp. 3018- 3025 ,(1982) , 10.1016/S0021-9258(19)81067-0
Bruce G. Adams, Induction of Galactokinase in Saccharomyces cerevisiae: Kinetics of Induction and Glucose Effects Journal of Bacteriology. ,vol. 111, pp. 308- 315 ,(1972) , 10.1128/JB.111.2.308-315.1972
R.J. Bram, N.F. Lue, R.D. Kornberg, A GAL family of upstream activating sequences in yeast: roles in both induction and repression of transcription. The EMBO Journal. ,vol. 5, pp. 603- 608 ,(1986) , 10.1002/J.1460-2075.1986.TB04253.X
Fred Sherman, Gerald R. Fink, James B. Hicks, Methods in yeast genetics Cold Spring Harbor Laboratory. ,(1979)
Jeffrey H Miller, Experiments in molecular genetics ,(1972)
Allan M. Maxam, Walter Gilbert, [57] Sequencing end-labeled DNA with base-specific chemical cleavages Nucleic Acids Part I. ,vol. 65, pp. 499- 560 ,(1980) , 10.1016/S0076-6879(80)65059-9
Yasuji Oshima, Regulatory Circuits for Gene Expression: The Metabolism of Galactose and Phosphate Cold Spring Harbor Monograph Archive. pp. 159- 180 ,(1982) , 10.1101/087969180.11B.159
K Matsumoto, A Toh-e, Y Oshima, Isolation and characterization of dominant mutations resistant to carbon catabolite repression of galactokinase synthesis in Saccharomyces cerevisiae. Molecular and Cellular Biology. ,vol. 1, pp. 83- 93 ,(1981) , 10.1128/MCB.1.2.83
M Johnston, R W Davis, Sequences that regulate the divergent GAL1-GAL10 promoter in Saccharomyces cerevisiae. Molecular and Cellular Biology. ,vol. 4, pp. 1440- 1448 ,(1984) , 10.1128/MCB.4.8.1440
R W West, R R Yocum, M Ptashne, Saccharomyces cerevisiae GAL1-GAL10 divergent promoter region: location and function of the upstream activating sequence UASG. Molecular and Cellular Biology. ,vol. 4, pp. 2467- 2478 ,(1984) , 10.1128/MCB.4.11.2467