Complete amino acid sequence of glucose dehydrogenase from Bacillus megaterium

作者: Klaus-Dieter Jany , Wolfgang Ulmer , Marion Fröschle , Gerhard Pfleiderer

DOI: 10.1016/0014-5793(84)80003-4

关键词: TyrosinePeptide sequenceBiochemistryBacillus megateriumHistidineBiologyEdman degradationAmino acidGlucose dehydrogenaseTryptophan

摘要: The amino acid sequence of glucose dehydrogenase from Bacillus megaterium has been determined. enzyme consists 4 identical subunits, each containing 262 residues. Its structure was established using manual Edman degradation procedures after modification the in native form with reagents specific to acids histidine, tyrosine, tryptophan and lysine order identify residues involved catalysis or located subunit binding area.

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