Molecular Strategies in the Analysis of the Porcine Genome

摘要: The main goal of genome research in livestock is to map and characterize trait loci controlling various phenotypic characters like disease resistance, reproduction other production traits economic importance (e.g. meat quality). Although identification mapping functional genes quantitative locus (QTL) regions associated with interest increased significantly pigs, the number mapped still small compared human mouse. Comparative analysis bioinformatic tools combined genomic post-genomic techniques enable achieve greater progress porcine genome. present thesis focuses on structural phosphoglycerate kinase (PGK) family which have previously been identified as candidate for inherited male fertility along disorders second part describes isolation microsatellite markers either from specific chromosomal region interest, or large-insert library clones harboring targeted genes, application these informative phylogenetic studies.The PGK i.e. ubiquitously expressed X-linked PGK1 gene autosomally determined testis-specific PGK2 gene, were SSCXq12-q13 SSC7q14-q15, respectively. 1665 bp full-length cDNA compiled using modified rapid amplification 5 -RACE 3 information. results sequences demonstrated that it a single-exon intronless complete open reading frame 1251 encoding protein 417 amino acids. Real time PCR showed mRNA was solely testis. There lower amount expression testis herniated boar very an 8-week-old piglet adult boar. Two SNPs (SNP-A: T427C; SNP-B: C914A) resulting acid substitutions Ser102-Pro102; Thr264-Lys264) detected genotyped among six pig breeds. SNP-A mutation proline could lead loss casein II (CK2) phosphorylation site peptide. Association analyses between some sperm quantity quality performed. SNP-B has significant effect semen volume Pietrian breed (p = 0.08).Fifteen (GT)n microsatellites (S0701 S0715) isolated nine PAC by improved efficient flanking sequence-based approach. Eight newly developed gene-associated, seven within poorly covered chromosome SSC16q refine this region. panel 245 unrelated animals representing 15 European Chinese indigenous A set eleven total 136 alleles used analyze relationships populations. Breed differentiation shown high average fixation index (FST 0.271). Neighbor-joining (NJ) tree unweighted pair group method arithmetic mean (UPGMA) dendrogram constructed. revealed breeds had diverged into two distinct groups, apart German Gottingen Minipig wild boar, clustered branches