Partitioning of plasmid R1. The ParM protein exhibits ATPase activity and interacts with the centromere-like ParR-parC complex
作者： Rasmus Bugge Jensen , Kenn Gerdes
关键词: Protein–protein interaction 、 Actin 、 Plasmid partitioning 、 Hexokinase 、 ParM 、 ATPase 、 Mutant 、 Biology 、 Biochemistry 、 Plasmid
摘要: The parA system of plasmid R1 consists two genes, parM and parR, a cis-acting centromere-like site parC. ParM protein exhibits similarity with superfamily ATPases that includes actin, hsp70 hexokinase. was purified to near-homogeneity assayed for in vitro ATPase activity. wild-type found posses Mutant derivatives exhibited decreased activity were non-functional vivo, indicating the ATP turnover by is essential correct partitioning. mutant proteins trans-dominance, suggesting participates as structural component partitioning apparatus. activated slightly presence ParR much greater extent when bound parC region. An analysis using yeast two-hybrid indicated interact, demonstrated interacts itself. Thus our results suggest direct interaction at natural partition parC, specifically stimulated this interaction.
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