The complete amino acid sequence of R-phycocyanin-I alpha and beta subunits from the red alga Porphyridium cruentum. Structural and phylogenetic relationships of the phycocyanins within the phycobiliprotein families.
作者： Axel DUCRET , Walter SIDLER , Gerhard FRANK , Herbert ZUBER
DOI: 10.1111/J.1432-1033.1994.TB18769.X
关键词: Phycoerythrocyanin 、 Biochemistry 、 Phycobiliprotein 、 Phycocyanobilin 、 Peptide sequence 、 Phycoerythrobilin 、 Biology 、 Porphyridium cruentum 、 Phycoerythrin 、 Phycocyanin
摘要: We present here the complete primary structure of R-phycocyanin-I alpha and beta subunits from red alga Porphyridium cruentum. The chain is composed 162 amino acid residues (18049 Da, calculated sequence, including chromophore) carries a phycocyanobilin pigment covalently linked to Cys84. contains 172 acids (19344Da, chromophores) at Cys82 phycoerythrobilin Cys153. A gamma-N-methyl asparagine residue was also characterised position 72 similar other phycobiliprotein subunits. cruentum shares high sequence identity with C-phycocyanins (69-83%), R-phycocyanins (66-70%) in less extent phycoerythrocyanins (57-65%) various sources. presented phylogenetic trees are based on comparison all sequences known so far confirm clear affiliation phycocyanin family. In spite their particular phycobilin pattern, they do not represent intermediate forms between phycoerythrin Phycoerythrocyanin, phycocyanin-related adapted green light harvesting, shown belong However, diverge phycocyanins different function it suggested that should be assigned separate group within
sci-hub.se 参考文章(84)
W.R. Belknap, R. Haselkorn, Cloning and light regulation of expression of the phycocyanin operon of the cyanobacterium Anabaena. The EMBO Journal. ,vol. 6, pp. 871- 884 ,(1987) , 10.1002/J.1460-2075.1987.TB04833.X
Gerhard Frank, New Aspects in Isocratic HPLC Separation of Phenylthiohydantoin Amino Acids Through the Application of Ionic Detergents Methods in Protein Sequence Analysis. pp. 116- 121 ,(1989) , 10.1007/978-3-642-73834-0_15
P. Freidenreich, G.S. Apell, A.N. Glazer, Structural studies on phycobiliproteins II. C-phycocyanin: amino acid sequence of the beta subunit. Specific cleavage of the alpha subunit. Journal of Biological Chemistry. ,vol. 253, pp. 212- 219 ,(1978) , 10.1016/S0021-9258(17)38290-X
Barbara A. Zilinskas, Dawn A. Howell, Comparative Immunology of the Phycobilisome Linker Polypeptides Progress in Photosynthesis Research. pp. 161- 164 ,(1987) , 10.1007/978-94-009-3535-8_39
Steven A. Cohen, Thomas L. Tarvin, Brian A. Bidlingmeyer, Amino Acid Analysis Using Pre-Column Derivatization with Phenylisothiocyanate: Matrix Effects and Tryptophan Analysis Springer, Boston, MA. pp. 207- 213 ,(1987) , 10.1007/978-1-4613-1787-6_22
C.H.W. Hirs, [20] Reduction and S-carboxymethylation of proteins Methods in Enzymology. ,vol. 11, pp. 199- 203 ,(1967) , 10.1016/S0076-6879(67)11022-7
Stanford Moore, William H. Stein, [117] Chromatographic determination of amino acids by the use of automatic recording equipment Methods in Enzymology. ,vol. 6, pp. 819- 831 ,(1963) , 10.1016/0076-6879(63)06257-1
Angelo Fontana, [34] Modification of tryptophan with BNPS-skatole (2-(2-nitrophenylsulfenyl)-3-methyl-3-bromoindolenine) Methods in Enzymology. ,vol. 25, pp. 419- 423 ,(1972) , 10.1016/S0076-6879(72)25037-6
Erhard Gross, [27] The cyanogen bromide reaction Methods in Enzymology. ,vol. 11, pp. 238- 255 ,(1967) , 10.1016/S0076-6879(67)11029-X
V Capuano, A S Braux, N Tandeau de Marsac, J Houmard, The "anchor polypeptide" of cyanobacterial phycobilisomes. Molecular characterization of the Synechococcus sp. PCC 6301 apce gene. Journal of Biological Chemistry. ,vol. 266, pp. 7239- 7247 ,(1991) , 10.1016/S0021-9258(20)89636-7