Identification of the promoter region of the human betaIGH3 gene.

摘要: PURPOSE To isolate and characterize the promoter of human betaIGH3 gene. METHODS Primer extension CapSite Hunting methods were used to determine transcription start sites (TSS) Putative factor-binding potential regions identified by online tools. Two clones containing 3 Kb 1 5'-flanking region gene isolated their respective activities characterized. Various fusion constructs promoter-luciferase reporter made transfect A549 cells. The responses these fragments TGF-beta1 also measured after being treated with at different concentrations. Several nonhuman cell lines transfected promoter-reporter construct compare activity in RESULTS site mRNA was determined be 65 bp upstream ATG codon. Both (-3011 -1) (-1000 displayed strong comparable Truncation analyses cells nucleotide from -336 -1 as having high (minimal promoter). results indicated that fragment -1000 -646 contained negative regulatory elements. Twenty ng/ml upregulated construct, but did not upregulate suggesting responsive elements existed -336. universally demonstrated all tested. CONCLUSIONS We a distinct pattern Further elucidation functions this may facilitate understanding its related corneal dystrophies.