IL-23 Promotes a Coordinated B Cell Germinal Center Program for Class-Switch Recombination to IgG2b in BXD2 Mice.
作者： Huixian Hong , Min Gao , Qi Wu , PingAr Yang , Shanrun Liu
关键词: Downregulation and upregulation 、 B cell 、 Population 、 Plasma cell 、 Molecular biology 、 Immunoglobulin class switching 、 Chemistry 、 Germinal center 、 Transcriptome 、 T helper cell
摘要: IL-23 promotes autoimmune disease, including Th17 CD4 T cell development and autoantibody production. In this study, we show that a deficiency of the p19 component in BXD2 (BXD2-p19-/- ) mouse leads to shift follicular helper program from (Tfh)-IL-17 Tfh-IFN-γ. Although germinal center (GC) size number GC B cells remained same, BXD2-p19-/- mice exhibited lower class-switch recombination (CSR) cells, leading serum levels IgG2b. Single-cell transcriptomics analysis revealed whereas Ifngr1, Il21r, Il4r genes synchronized expression pattern with Cxcr5 plasma genes, Il17ra Cxcr4 genes. Downregulation Ighg2b was associated decreased CSR-related novel base excision repair were otherwise predominantly expressed by + mice. Together, these results suggest although is dispensable for formation, it essential promote population Tfh-IL-17 cells. acts indirectly on facilitate during dark zone phase development.
sci-hub.st 参考文章(74)
David P. Harris, Stephen Goodrich, Katja Mohrs, Markus Mohrs, Frances E. Lund, Cutting Edge: The Development of IL-4-Producing B Cells (B Effector 2 Cells) Is Controlled by IL-4, IL-4 Receptor α, and Th2 Cells Journal of Immunology. ,vol. 175, pp. 7103- 7107 ,(2005) , 10.4049/JIMMUNOL.175.11.7103
Shutao Xie, Hui-Chen Hsu, John D Mountz, John H Wang, Cytokine regulation of B-cell migratory behavior favors formation of germinal centers in autoimmune disease. Discovery Medicine. ,vol. 11, pp. 76- 85 ,(2011)
John H. Wang, James S. New, Shutao Xie, PingAr Yang, Qi Wu, Jun Li, Bao Luo, Yanna Ding, Kirk M. Druey, Hui-Chen Hsu, John D Mountz, Extension of the Germinal Center Stage of B-cell Development Promotes Autoantibodies in BXD2 Mice Arthritis & Rheumatism. ,vol. 65, pp. 2703- 2712 ,(2013) , 10.1002/ART.38059
Shutao Xie, Jun Li, John H. Wang, Qi Wu, PingAr Yang, Hui-Chen Hsu, Lesley E. Smythies, John D. Mountz, IL-17 Activates the Canonical NF-κB Signaling Pathway in Autoimmune B Cells of BXD2 Mice To Upregulate the Expression of Regulators of G-Protein Signaling 16 Journal of Immunology. ,vol. 184, pp. 2289- 2296 ,(2010) , 10.4049/JIMMUNOL.0903133
Yanna Ding, Jun Li, PingAr Yang, Bao Luo, Qi Wu, Allan J. Zajac, Oliver Wildner, Hui-Chen Hsu, John D. Mountz, Interleukin-21 promotes germinal center reaction by skewing the follicular regulatory T cell to follicular helper T cell balance in autoimmune BXD2 mice. Arthritis & Rheumatism. ,vol. 66, pp. 2601- 2612 ,(2014) , 10.1002/ART.38735
Stephen M. Shinall, Mercedes Gonzalez-Fernandez, Randolph J. Noelle, Thomas J. Waldschmidt, Identification of murine germinal center B cell subsets defined by the expression of surface isotypes and differentiation antigens. Journal of Immunology. ,vol. 164, pp. 5729- 5738 ,(2000) , 10.4049/JIMMUNOL.164.11.5729
Hui-Chen Hsu, Yalei Wu, PingAr Yang, Qi Wu, Godwin Job, Jian Chen, John Wang, Mary Ann V. Accavitti-Loper, William E. Grizzle, Robert H. Carter, John D. Mountz, Overexpression of Activation-Induced Cytidine Deaminase in B Cells Is Associated with Production of Highly Pathogenic Autoantibodies Journal of Immunology. ,vol. 178, pp. 5357- 5365 ,(2007) , 10.4049/JIMMUNOL.178.8.5357
John H. Wang,, Jun Li, Qi Wu, PingAr Yang, Rahul D. Pawar, Shutao Xie, Laura Timares, Chander Raman, David D. Chaplin, Lu Lu, John D. Mountz, Hui-Chen Hsu, Marginal zone precursor B cells as cellular agents for type I IFN-promoted antigen transport in autoimmunity. Journal of Immunology. ,vol. 184, pp. 442- 451 ,(2010) , 10.4049/JIMMUNOL.0900870
Hao Li, Hui-Chen Hsu, Qi Wu, PingAr Yang, Jun Li, Bao Luo, Mohamed Oukka, Claude H. Steele, Daniel J. Cua, William E. Grizzle, John D. Mountz, IL-23 promotes TCR-mediated negative selection of thymocytes through the upregulation of IL-23 receptor and RORγt Nature Communications. ,vol. 5, pp. 4259- 4259 ,(2014) , 10.1038/NCOMMS5259
Elissa K. Deenick, Jhagvaral Hasbold, Philip D. Hodgkin, Decision criteria for resolving isotype switching conflicts by B cells. European Journal of Immunology. ,vol. 35, pp. 2949- 2955 ,(2005) , 10.1002/EJI.200425719