Regulation of tyrosine hydroxylase activity and phosphorylation at Ser19 and Ser40 via activation of glutamate NMDA receptors in rat striatum
作者： Niklas Lindgren , Zhi-Qing David Xu , Maria Lindskog , Mario Herrera-Marschitz , Michel Goiny
DOI: 10.1046/J.1471-4159.2000.0742470.X
关键词: Tyrosine 3-Monooxygenase 、 Glutamate receptor 、 Forskolin 、 NMDA receptor 、 Dopamine 、 Adenylyl cyclase 、 Internal medicine 、 Biology 、 Endocrinology 、 Phosphorylation 、 Tyrosine hydroxylase
摘要: The activity of tyrosine hydroxylase, the rate-limiting enzyme in biosynthesis dopamine, is stimulated by phosphorylation. In this study, we examined effects activation NMDA receptors on state phosphorylation and hydroxylase rat striatal slices. produced a time-and concentration-dependent increase levels phospho-Ser(19)-tyrosine nigrostriatal nerve terminals. This was not associated with any changes basal measured as DOPA accumulation. Forskolin, an activator adenylyl cyclase, at Ser(40) caused significant reduced forskolin-mediated increases both addition, phospho-Ser(40)-tyrosine okadaic acid, inhibitor protein phosphatase 1 2A, but cyclic AMP analogue, 8-bromo-cyclic AMP. These results indicate that, striatum, glutamate decreases via reducing production. They also provide mechanism for demonstrated ability to decrease dopamine synthesis.
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