Identification of rose cultivars by restriction fragment length polymorphism
作者： Sriyani Rajapakse , Mark Hubbard , John W. Kelly , Albert G. Abbott , Robert E. Ballard
DOI: 10.1016/0304-4238(92)90024-7
关键词: Genetic marker 、 HindIII 、 Restriction fragment length polymorphism 、 Biology 、 Southern blot 、 Genomic library 、 Genetics 、 Low copy number 、 Restriction enzyme 、 genomic DNA
摘要: Abstract Restriction fragment length polymorphisms (RFLP) were investigated as a means of identifying rose ( Rosa × hybrida) cultivars for patent protection. A library genomic DNA was generated by shotgun cloning HindIII digested fragments the cultivar ‘Confection’ into plasmid pUC8. Clones with low copy number sequences identified and used probes in Southern hybridization analyses restriction from different cultivars. We have 15 that display RFLP useful identification. One these probes, pH1.3C, can identify 12 Based on hybridizations pH1.3C pH1.6C, total 16 been distinguished one another. Using enzyme probe combinations, we expect to distinguish many more
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