Dopamine concentration in the cytoplasmic compartment of single neurons determined by capillary electrophoresis
作者： Teresa M. Olefirowicz , Andrew G. Ewing
DOI: 10.1016/0165-0270(90)90036-F
关键词: Cell 、 Dopamine 、 Cytoplasm 、 Neurotransmitter 、 Neuroscience 、 Biology 、 Nervous system 、 Cellular neuroscience 、 Biophysics 、 Compartmentalization (fire protection) 、 Capillary electrophoresis
摘要: It has long been a goal of the biochemist to study chemistry single cells. This is particularly important in neuroscience, since each cell can maintain specific function and identity. Investigations into neurotransmitter compartmentalization within body neurons should lead an understanding role metabolism, uptake storage neurotransmitters. Using capillary electrophoresis, it possible separate detect attomole levels picoliter volumes cytoplasm withdrawn from pond snail, Planorbis corneus. work demonstrates, for first time, direct determination cytoplasmic concentration dopamine single, intact neurons.
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