On the fidelity of DNA replication. Lack of primer position effect on the fidelity of mammalian DNA polymerases.
作者： J Abbotts , L A Loeb
DOI: 10.1016/S0021-9258(17)39786-7
关键词: Position effect 、 Primer (molecular biology) 、 DNA polymerase 、 Genetics 、 DNA 、 Proofreading 、 DNA replication 、 Fidelity 、 Processivity 、 Biology
摘要: Abstract Mechanisms for the fidelity of DNA replication in eucaryotes are not adequately understood. Certain hypotheses can be tested by examining whether first nucleotide inserted is incorporated with a significantly higher error rate than subsequent nucleotides. Using synthetic oligodeoxynucleotides, we have measured effect primer position on single-base misinsertion frequencies at an amber site phi X174 DNA. Our results show lack effect, indicating that processivity and most direct "energy relay" proofreading mechanisms important determinants eucaryotic fidelity.
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