tRNA binding with anti-cancer alkaloids-nature of interaction and comparison with DNA-alkaloids adducts.

摘要: Abstract Vincristine and vinblastine are potent anti-proliferative compound whose mechanism of action inside a cell is not well elucidated the basis their differential cellular effect also unknown. This work focuses towards understanding interaction vincristine with tRNA using spectroscopic approach. Fourier transform infrared (FTIR) spectroscopy, difference spectroscopy UV–visible were used to study binding parameters tRNA–alkaloids interaction. Both vinca alkaloids interact through external some degree intercalation into nitrogenous bases. The adduct formation did alter A-conformation biopolymer vincristine–tRNA complexes found be more stable than that vinblastine–tRNA complexes. constants (K) estimated for VCR–tRNA VBS–tRNA complexation 3 × 102 M−1 2.5 × 102 M−1 respectively, which suggests low affinity tRNA. recognizes properties vital contributes better could help in identifying reason behind diverse cell.