Nucleotide sequence of cloned cDNA for human pancreatic kallikrein

摘要: Cloned cDNA sequences for human pancreatic kallikrein have been isolated and determined by molecular cloning sequence analysis. The identity between urinary kallikreins is indicated the complete coincidence amino acid deduced from cloned that reported partially kallikrein. active enzyme form of consists 238 acids preceded a signal peptide profragment 24 acids. A comparison this with other mammalian indicates key residues required both serine protease activity kallikrein-like cleavage specificity are retained in sequence, corresponding to some external loops diverge kallikreins. Analyses RNA blot hybridization, primer extension, S1 nuclease mapping indicate mRNA also expressed kidney sublingual gland, suggesting synthesis these tissues. Furthermore, tissue-specific regulation expression members gene family has discussed.