FURTHER STUDIES OF AN INHIBITOR OF THE TWO ANTIBODY IMMUNOASSAY SYSTEM.

作者: C. R. Morgan , R. L. Sorenson , A. Lazarow

DOI: 10.2337/DIAB.13.6.579

关键词: ChemistryGamma globulinMolecular biologyImmunochemistryImmunoelectrophoresisAntigen-antibody reactionsImmunoassay methodImmunoassayChromatographyAntibody

摘要: Previous studies with the two antibody immunoassay method have demonstrated presence of an “inhibitor” in fresh plasma which interferes precipitating step system. Immunoelectrophoretic described here no cross-reactivity rat or human gamma globulin anti-guinea pig (rabbit source), had been suggested by other workers. Concomitant inactivation complement and heat, antigen-antibody complex, EDTA, ammonia indicates that is inhibitor EDTA (0.01 M ) inactivates “inhibitor,” permitting assay undiluted method.

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