作者: C. R. Morgan , R. L. Sorenson , A. Lazarow
关键词: Chemistry 、 Gamma globulin 、 Molecular biology 、 Immunochemistry 、 Immunoelectrophoresis 、 Antigen-antibody reactions 、 Immunoassay method 、 Immunoassay 、 Chromatography 、 Antibody
摘要: Previous studies with the two antibody immunoassay method have demonstrated presence of an “inhibitor” in fresh plasma which interferes precipitating step system. Immunoelectrophoretic described here no cross-reactivity rat or human gamma globulin anti-guinea pig (rabbit source), had been suggested by other workers. Concomitant inactivation complement and heat, antigen-antibody complex, EDTA, ammonia indicates that is inhibitor EDTA (0.01 M ) inactivates “inhibitor,” permitting assay undiluted method.