Tyrosine phosphorylation of a 22-kDa protein is correlated with transformation by Rous sarcoma virus.
作者： J.R. Glenney
DOI: 10.1016/S0021-9258(19)47038-5
关键词: Tyrosine phosphorylation 、 Protein phosphorylation 、 Molecular biology 、 SH2 domain 、 Receptor tyrosine kinase 、 Tyrosine kinase 、 Protein tyrosine phosphatase 、 Biology 、 Phosphorylation 、 Proto-oncogene tyrosine-protein kinase Src
摘要: Recent studies from this laboratory have identified novel cytoskeletal proteins that are phosphorylated on tyrosine in vivo Rous sarcoma virus-transformed chick fibroblasts (Glenney, J. R., Jr., and Zokas, L. (1989) Cell Biol. 108, 2401-2408). In the present report, phosphorylation of these was examined cells expressing nonmyristylated mutants src not transformed. A good correlation found between transformation a 22-kDa protein. Tyrosine protein reduced more than 95% src. Size fractionation revealed phosphoprotein transformed is Mr 150,000 complex. Monoclonal antibodies were used to screen various chicken tissues where at high levels muscle lung with low epithelial brain. The becomes an excellent candidate for mediator by kinase class oncogenes.
sci-hub.st 参考文章(22)
G Peaucellier, P A Veno, W H Kinsey, Protein tyrosine phosphorylation in response to fertilization. Journal of Biological Chemistry. ,vol. 263, pp. 13806- 13811 ,(1988) , 10.1016/S0021-9258(18)68315-2
A Wolfman, TG Wingrove, PJ Blackshear, IG Macara, Down-regulation of protein kinase C and of an endogenous 80-kDa substrate in transformed fibroblasts. Journal of Biological Chemistry. ,vol. 262, pp. 16546- 16552 ,(1987) , 10.1016/S0021-9258(18)49290-3
T W Wong, A R Goldberg, In vitro phosphorylation of angiotensin analogs by tyrosyl protein kinases. Journal of Biological Chemistry. ,vol. 258, pp. 1022- 1025 ,(1983) , 10.1016/S0021-9258(18)33153-3
Michinari Hamaguchi, Carla Grandori, Hidesaburo Hanafusa, None, Phosphorylation of cellular proteins in Rous sarcoma virus-infected cells: analysis by use of anti-phosphotyrosine antibodies. Molecular and Cellular Biology. ,vol. 8, pp. 3035- 3042 ,(1988) , 10.1128/MCB.8.8.3035
A B Reynolds, D J Roesel, S B Kanner, J T Parsons, Transformation-specific tyrosine phosphorylation of a novel cellular protein in chicken cells expressing oncogenic variants of the avian cellular src gene. Molecular and Cellular Biology. ,vol. 9, pp. 629- 638 ,(1989) , 10.1128/MCB.9.2.629
F R Cross, E A Garber, D Pellman, H Hanafusa, A short sequence in the p60src N terminus is required for p60src myristylation and membrane association and for cell transformation. Molecular and Cellular Biology. ,vol. 4, pp. 1834- 1842 ,(1984) , 10.1128/MCB.4.9.1834
E. B. Pasquale, P. A. Maher, S. J. Singer, Talin is phosphorylated on tyrosine in chicken embryo fibroblasts transformed by Rous sarcoma virus. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 83, pp. 5507- 5511 ,(1986) , 10.1073/PNAS.83.15.5507
J. R. Glenney, B. F. Tack, Amino-terminal sequence of p36 and associated p10: identification of the site of tyrosine phosphorylation and homology with S-100. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 82, pp. 7884- 7888 ,(1985) , 10.1073/PNAS.82.23.7884
M. P. Kamps, J. E. Buss, B. M. Sefton, Mutation of NH2-terminal glycine of p60src prevents both myristoylation and morphological transformation. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 82, pp. 4625- 4628 ,(1985) , 10.1073/PNAS.82.14.4625
E Erikson, Identification of a cellular protein substrate phosphorylated by the avian sarcoma virus-transforming gene product. Cell. ,vol. 21, pp. 829- 836 ,(1980) , 10.1016/0092-8674(80)90446-8