Identification of the Zebrafish IFN Receptor: Implications for the Origin of the Vertebrate IFN System

摘要: The recent description of virus-induced fish IFNs has raised questions about the evolution this complex antiviral system. Identification receptor zebrafish IFN (zIFN) was sought to help resolve these questions. We set up an experimental system study zIFN in course a viral infection embryos. In setting, induced by infection, and we identified zIFN-dependent transcripts. Embryos quickly died from but overexpression increased their survival. took advantage perform vivo loss gain function analysis candidate receptors class II helical family zCRFB1 zCRFB5 as two subunits receptor. Based on organization gene protein structure components, appear orthologs mammalian IFN-lambda, specifying type III ancestral vertebrates.