Surface acoustic wave biosensor based on odorant binding proteins deposited by laser induced forward transfer
作者： Fabio Di Pietrantonio , Massimiliano Benetti , Domenico Cannata , Antonio Varriale , Sabato D'Auria
关键词: Fluence 、 Surface acoustic wave sensor 、 Resonator 、 Biosensor 、 Laser 、 Nitrogen 、 Materials science 、 Odorant binding 、 Analytical chemistry 、 Optics 、 Surface acoustic wave
摘要: In this paper, a biosensor for vapor phase detection of odorant molecules based on Surface Acoustic Wave (SAW) resonators is presented. The SAW were coated with wild-type odorant-binding protein from pig (wtpOBP) deposited by laser-induced forward transfer (LIFT). Two solutions, i.e. 20% and 50% (v/v) glycerol, LIFT changing the laser fluence printing morphology droplets. was tested in nitrogen upon exposure to R-(-)-1-octen-3-ol (octenol) R-(-)-carvone (carvone) vapors range concentrations between 9 60 ppm. An uncoated device used compensate responses due variations environmental parameters. differential response curves showed linear behavior obtained sensitivities 20.7 Hz/ppm 13.8 Hz/ppm, respectively, octenol carvone.
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