Methods for Studying DNA Single-Strand Break Repair and Signaling in Xenopus laevis Egg Extracts.
作者： Yunfeng Lin , Anh Ha , Shan Yan
DOI: 10.1007/978-1-4939-9500-4_9
关键词: DNA damage 、 DNA Repair Pathway 、 Cell biology 、 Biology 、 DNA replication 、 DNA Single Strand Break 、 Xenopus 、 Genome instability 、 DNA 、 Plasmid
摘要: DNA single-strand breaks (SSBs) are the most common type of lesions as they generated approximately 10,000 times per mammalian cell each day. Unrepaired SSBs compromise replication and transcription programs, leading to genome instability, have been implicated in many diseases including cancer. In this chapter, we introduce methods study ATR-Chk1 damage response (DDR) pathway repair a site-specific, defined SSB plasmid Xenopus laevis egg extracts. This experimental system can be applied future studies reveal aspects molecular mechanisms signaling eukaryotes.
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