One-pot, green synthesis of gold nanoparticles by gelatin and investigation of their biological effects on Osteoblast cells.

摘要: Abstract It is useful to find new methods synthesize and, more importantly, control the size and shape of gold nanoparticles (AuNPs) without using relatively toxic-reducing agents surfactants. In this work, we present a one-pot, green synthesis AuNPs taking advantage gelatin biopolymer operate as unique reducing, growth controlling stabilizing agent in aqueous solution tetrachloroauric acid (HAuCl4) at temperatures above its melting point (∼35 °C). The were found be strongly influenced by concentration (0.5–5%), while rate controlled temperature (40–80 °C) viscosity biopolymer. A specific class gelatin-coated was selected investigate stability simulated physiological conditions cellular media subsequently evaluate vitro biocompatibility capacity sustain proliferation differentiation Osteoblast cells. Dark-field microscopy Rayleigh scattering spectra prove efficient internalization compared with citrate-coated AuNPs, methylthiazoltetrazolium bromide (MTT) assay demonstrates enhanced cell proliferation. Interestingly, presence out first sign cells bone nodules formation, confirmed alkaline phosphatase (ALP) activity assay.