Enzymatic biofuel cell-based self-powered biosensing of protein kinase activity and inhibition via thiophosphorylation-mediated interface engineering
作者： Chengcheng Gu , Panpan Gai , Lei Han , Wen Yu , Qingyun Liu
DOI: 10.1039/C8CC02328J
关键词: Biosensor 、 Enzymatic biofuel cell 、 Protein kinase A 、 Interface engineering 、 Chemistry 、 Biochemistry 、 Detection limit 、 A549 cell
摘要: We developed a facile and ultrasensitive enzymatic biofuel cell (EBFC)-based self-powered biosensor of protein kinase A (PKA) activity inhibition via thiophosphorylation-mediated interface engineering. The detection limit was down to 0.00022 U mL−1 (S/N = 3). In addition, the PKA activities from MCF-7 A549 lysates were analyzed achieved reliable results.
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